I-Gel Yellow Blood Collection Tube

Amashubhu okuqoqwa kwejeli okuhlukanisa igazi asetshenziswe kabanzi kumalabhorethri emtholampilo.Ijeli ehlukanisayo ingenza ungqimba lokuzihlukanisa phakathi kwezingxenye zeseli kanye ne-serum (i-plasma), ivimbele ngempumelelo ukushintshana kwezinto phakathi kwamangqamuzana egazi ne-serum (i-plasma), futhi iqinisekise ukuqina kwezingxenye ze-serum (plasma) phakathi nenkathi ethile yesikhathi.Iglue ehlukanisayo ikakhulukazi yakhiwe ngerabha ye-silicone, ama-macromolecular hydrocarbons, iglue ye-hydrophobic, njll. Njengempahla ye-polymer, ayincibiliki emanzini futhi inert.Iwuketshezi lwe-thixotropic viscous olunobuningi obungu-1.04-1.05 mmol/ Phakathi kwe-L, lunobuhle bokumelana ne-oxidation, ukumelana nezinga lokushisa eliphezulu, ukumelana nezinga lokushisa eliphansi, nokuqina komoya okuhle.Ukuminyana kwe-serum ngu-1.026-1.031 mmol/L, kanti i-hematocrit ingu-1.090-1.095.Ngenxa yamandla adonsela phansi athile, ijeli ehlukanisayo iphakathi nje kwe-serum namaseli egazi, ngakho ngaphansi kwezimo ezivamile, igazi lizovela ngokulandelana ngemva kwe-centrifugation.I-Serum, ijeli ehlukanisayo, namaseli egazi 3 phansi.

Ngokuvamile kunezinhlobo ezimbili zamashubhu okuqoqa igazi ejeli avame ukusetshenziswa kulabhorethri: ishubhu lokuhlukanisa ijeli ye-serum procoagulation kanye ne-plasma yokuhlukanisa ijeli ye-anticoagulation tube.Ishubhu ye-serum yokuhlukanisa ijeli ye-procoagulation iwukwengeza i-coagulant eshubhuni lokuqoqwa kwegazi ukuze kufinyezwe isikhathi sokuhlangana kwegazi, ithole i-serum ngokushesha, futhi ibike imiphumela ngesikhathi esifushane kakhulu.Amashubhu eqoqo legazi lengilazi awadingi ukwengeza ama-coagulant, futhi igazi elithinta udonga lweshubhu lengilazi lizodala ukuhlangana.Kodwa-ke, lapho izici zokuhlangana kwe-XI ne-XII zihlangana namashubhu epulasitiki wokuqoqa igazi, amandla awo okwenziwa asebenze abuthakathaka kakhulu, futhi i-coagulant idinga ukungezwa ukuze kufinyezwe isikhathi sokuhlangana.Ishubhu yokuhlukanisa i-plasma ye-anticoagulation ifafazwa ngama-anticoagulant afana ne-lithium heparin odongeni lwangaphakathi lweshubhu lokuqoqa igazi lejeli ukuze kuhlangatshezwane nezidingo zokuhlolwa okuphuthumayo kwe-plasma kwamakhemikhali ezinto eziphilayo.

Ekusetshenzisweni okungokoqobo, kuvame ukutholakala ukuthi umphumela wokuhlukanisa weshubhu lokuqoqa igazi lejeli awulungile, isibonelo: kwamanye amashubhu enjoloba ahlukene, kungabonakala ukuthi izingcezu zejeli zokuhlukanisa noma amaconsi kawoyela antanta ebusweni be-jeli. i-serum noma imisiwe ku-serum;ungqimba lwejeli lokuhlukanisa luntanta phezu kweserum.ngenhla njll. Ukuhlukanisa amajeli nakho kungaphazamisa eminye imiphumela yokuhlolwa.Emnyangweni wethu, kutholwe ukuthi iqoqo elithile lama-reagents kanye nejeli esheshayo yokuhlukanisa i-serum kusabelana ngesikhathi sokutholwa kwe-HBSAg yohlelo lokuthola i-Abbott i2000SR, okubangele imiphumela emihle engamanga.

Leli phepha ikakhulukazi lihlaziya kusuka ezicini ezimbili, okungukuthi, izizathu zomphumela omubi wokuhlukanisa wejeli ehlukanisayo, nomthelela wokwethulwa kwejeli ehlukanisayo esilinganisweni.

1. Indlela yokuhlukanisa i-serum ne-plasma ngokuhlukanisa ijeli Ijeli ehlukanisayo i-thixotropic mucocolloid eyakhiwe ngama-hydrophobic organic compounds kanye ne-silica powder.Isakhiwo siqukethe inani elikhulu lamabhondi e-hydrogen.Ukuba khona kwamabhondi e-hydrogen kwakha isisekelo samakhemikhali se-thixotropy yejeli ehlukanisayo..Amandla adonsela phansi athile ejeli ehlukanisayo agcinwa ku-1.05, amandla adonsela phansi athile engxenye yoketshezi lwegazi acishe abe ngu-1.02, futhi amandla adonsela phansi athile engxenye yegazi eyakhiwe cishe angu-1.08.Lapho ijeli ehlukanisayo kanye negazi elijiyile (noma igazi eligcwele i-anticoagulated) ku-centrifuged ku-tube yokuhlola efanayo, Ngenxa yamandla aphakathi nendawo asetshenziswa kujeli ehlukanisayo, isakhiwo senethiwekhi ye-hydrogen bond siphulwa sibe isakhiwo esifana neketango, futhi okuhlukanisayo. ijeli iba uketshezi olune-viscosity ephansi.Ngenxa yamandla adonsela phansi athile ahlukile, ijeli yokuhlukanisa iyahlehliswa futhi ihlukaniswe ukuze yakhe izingqimba ezintathu zehlule legazi (igazi elinqamula ukuhlangana)/okuhlukanisa ijeli/serum (i-plasma).Lapho i-centrifuge iyeka ukujikeleza futhi ilahlekelwa amandla e-centrifugal, izinhlayiya zeketanga kujeli ehlukanisayo zakha isakhiwo senethiwekhi futhi ngamabhondi e-hydrogen, zibuyisele isimo sejeli esine-viscosity ephezulu, futhi zakha ungqimba lokuzihlukanisa phakathi kwe-serum (i-plasma) nehlule legazi (i-anticoagulated). igazi eliphelele)..

2. Izizathu zomphumela omubi wokuhlukanisa wejeli yokuhlukanisa

2.1 Ukuhlukanisa Ikhwalithi Yejeli Amandla adonsela phansi athile ejeli ehlukanisayo aphakathi kwalawo e-serum (i-plasma) namangqamuzana egazi, okuyisisekelo esibonakalayo sokubuyiselwa emuva kwejeli ehlukanisayo kanye nokuhlukaniswa kwe-serum (i-plasma).Uma ikhwalithi yejeli yokuhlukanisa yeshubhu lokuqoqa igazi impofu futhi amandla adonsela phansi athile angahlangabezani nezidingo, nakanjani kuzothinta umphumela wokuhlukanisa i-serum (i-plasma), kanye nokwenzeka ukuthi ijeli yokuhlukanisa ne-serum (i-plasma) okuhlangene kungenzeka.

2.2 Ukujiya kwegazi okungaphelele Ngemva kwe-centrifugation, ngezinye izikhathi indawo yejeli yokuhlukanisa kanye namahlule e-serum namahlule egazi akuhlukanisiwe ngokuphelele, futhi imicu ye-fibrin ivela ku-serum.Isizathu ngokuvamile ukuthi igazi alijikijeli ngokugcwele ngaphambi kwe-centrifugation.Ukujiya kwegazi okungaphelele kungabangela ukuthi i-fibrin ixutshwe ongqimbeni lokuhlukanisa.Ishubhu lenjoloba lokuhlukanisa i-serum kufanele lisetshenziswe ngendlela efanele ngokwemiyalo, futhi i-serum ingalungiswa nge-centrifugation ngemva kokuba igazi selijiyile ngokuphelele (ngokuvamile, ishubhu lepulasitiki eliqukethe i-coagulant lidinga ukubekwa liqonde cishe imizuzu engama-30, negazi. ithubhu yokuqoqa ngaphandle kwe-coagulant idinga ukubekwa iqonde imizuzu engama-60-90).amasampula e-serum aphezulu.

2.3 Ukushisa kwe-Centrifugation Izinga lokushisa le-centrifugation linomthelela omkhulu emthelela wokuhlukanisa i-serum neshubhu yejeli yokuhlukanisa.I-serum yayicacile kushubhu ehlukanisayo yejeli esheshayo ehlukaniswe i-centrifuge evamile ekamelweni lokushisa, kodwa ubuhlalu obunamafutha obunosayizi abahlukene buvele ku-15% kuya ku-20% wamasampuli.Ngakolunye uhlangothi, abukho ubuhlalu obunamafutha atholakala ku-serum ehlukaniswe neshubhu lokuhlola eligxilwe yi-centrifuge ephansi yokushisa.Lapho izinga lokushisa lidlula izinga lokushisa lokugcina elidingekayo kujeli lokuhlukanisa, ijeli ye-inert izoncibilika ku-serum.Ngeke nje kuphela ivimbe futhi ingcolise inaliti yesampula nenkomishi yokusabela yokuhlaziya i-biochemical, kodwa futhi ibe nomthelela omkhulu kweminye imiphumela yokulinganisa kwamakhemikhali ezinto eziphilayo.