Ishubhu elimhlophe lokutholwa kwe-Nucleic Acid
Incazelo emfushane:
Isetshenziselwa ngokukhethekile ukutholwa kwe-nucleic acid, futhi ikhiqizwa ngokuphelele ngaphansi kwezimo zokuhlanzeka, okunciphisa ukungcola okungaba khona phakathi nenqubo yokukhiqiza futhi inciphise ngempumelelo umthelela wokungcoliseka okungase kube khona ekuhlolweni.
1. Ukuhlolwa kwevolumu yokumunca: Ivolumu yokumunca, okungukuthi, inani legazi elikhishiwe, linephutha phakathi kuka-±10%, ngaphandle kwalokho alifanelekile.Inani elingalungile legazi elidonswayo liyinkinga enkulu okwamanje.Lokhu akugcini nje ngokuphumela emiphumeleni yokuhlola engalungile, kodwa futhi kubangela ukuvala nokulimala kwemishini yokuhlola.
2. Ukuhlolwa kokuvuza kwesitsha: Ishubhu lokuqoqa igazi elingenalutho eliqukethe isisombululo esiyinhlanganisela ye-sodium fluorescein labekwa libheke phansi emanzini enziwe i-deionized imizuzu engu-60.Ngaphansi komthombo wokukhanya we-ultraviolet wegagasi elide, akukho fluorescence ebonwe ngaphansi kombono ojwayelekile ekamelweni elimnyama, elalifaneleka.Ukuvuza kwesiqukathi kuyisizathu esiyinhloko somthamo wegazi ongalungile weshubhu lamanje lokuqoqa igazi levacuum.
3. Ukuhlolwa kwamandla esitsheni: isiqukathi singaphansi kwe-centrifuge ene-centrifugal acceleration engu-3000g imizuzu engu-10, futhi siyafaneleka uma singadabuki.Izidingo eziqinile phesheya yilezi: amamitha ama-2 ngaphezu komhlaba, ishubhu lokuqoqa igazi levacuum liwa liqonde ngaphandle kokuphuka, elingavimbela ukulimala ngengozi kweshubhu lokuhlola nokulahlekelwa ama-specimens.
4. Isivivinyo sesikhala esincane samahhala: Isikhala esincane sokuqinisekisa ukuthi igazi lixutshwe ngokugcwele.Inani legazi elidonswayo ngu-0.5ml-5ml, >+25% yenani legazi elikhishiwe;uma inani legazi elidonswayo li>5ml,>15% yenani legazi elidonswayo.
5. Ukuhlolwa kokunemba kwe-solvent, i-solute mass ratio kanye nenani lokwengeza isixazululo: iphutha kufanele libe phakathi kuka-±10% weplanti evamile eshiwo.Lena inkinga enganakwa kalula futhi evamile, futhi ingesinye sezizathu eziyinhloko zedatha yokuhlola engalungile.
