Amashubhu we-PRP Acd
Incazelo emfushane:
I-Anticoagulant Citrate Dextrose Solution, evame ukubizwa ngokuthi i-ACD-A noma Isixazululo A iyisixazululo esingeyona i-pyrogenic, esiyinyumba.Lesi sici sisetshenziswa njenge-anticoagulant ekukhiqizeni i-plasma ecebile ngeplatelet (PRP) ene-PRP Systems yokucubungula igazi elingaphandle kwe-extracorporeal.
I-Anticoagulant Citrate Dextrose Solution, evame ukubizwa ngokuthi i-ACD-A noma Isixazululo A iyisixazululo esingeyona i-pyrogenic, esiyinyumba.Lesi sici sisetshenziswa njenge-anticoagulant ekukhiqizeni i-plasma ecebile ngeplatelet (PRP) ene-PRP Systems yokucubungula igazi elingaphandle kwe-extracorporeal.Ama-Anticoagulants asekelwe ku-citrate asebenzisa ikhono le-citrate ion ukuze chelate i-calcium ionized ekhona egazini ukuvimbela ukuhlangana kwegazi futhi enze inkimbinkimbi ye-calcium-citrate engeyona i-ionized.
Okuwukuphela komkhiqizo we-anticoagulant ogunyazwe i-United States Food & Drug Administration ukuthi usetshenziselwe ukulungiswa kwe-PRP kuma-PRP Systems ahlukahlukene yi-ACD-A.Ngokocwaningo olwenziwa ngo-2016 ku-PRP olutholwe ngama-anticoagulant ahlukahlukene kanye nemiphumela yawo ekuziphatheni kwamaseli e-mesenchymal stromal in vitro kanye nezinombolo zeplatelet, kunemiphumela emihle ekusetshenzisweni kwe-PRP yokulungisa izicubu ze-musculoskeletal.
Ukuhlukanisa ama-platelet kuyalulekwa ukuthi kufakwe esikhundleni se-sodium citrate ejwayelekile ye-Acid Citrate Dextrose (ACD-A) njengoba inqubo yokuhlukanisa idinga izinyathelo eziningi zokugeza.Ama-platelet azinze kakhulu ku-37C ngesikhathi sokuphothwa, kodwa ukuwaphotha ekamelweni lokushisa (25 C) nakho kusebenza kahle.Ukunciphisa i-pH nge-ACD-A (isondela ku-6.5) kusiza ukuphazamisa ukusebenza kwensalela ye-thrombin traces kumashubhu e-platelet, futhi kunomthelela ekugcinweni okuphelele kwe-platelet morphology ngenkathi kushintsha ukusebenza ku-minima.Ngokujwayelekile udinga ukumisa kabusha ama-platelet ku-Tyrode Buffer efanele (pH 7.4) ukuze ubuyisele ukusebenza kwawo.I-ACD inezinzuzo eziningi uma kuziwa ekulondolozeni ama-platelet
Lapho i-ACD isetshenziswa, imiphumela yabonisa isivuno esiphezulu seplatelet egazini lilonke.Kodwa-ke, ukusetshenziswa kwe-EDTA kuphinde kwakhuthaza ukukhula kwevolumu yeplatelet emaphakathi ngemva kokukhishwa kwezinyathelo ze-centrifugation yegazi ukuze kutholwe i-PRP.Okulandelayo, ukusetshenziswa kwe-ACD kubangele ukwanda kwamaseli e-mesenchymal stromal.Ngakho-ke, kwaphethwa ngokuthi ama-anticoagulant kuhlanganise ne-ACD-A adlala indima enkulu ekulungiseleleni i-PRP futhi asize ukuthuthukisa inqubo ngezinga elikhulu.
