I-Platelet-rich plasma (PRP) iwukuhlushwa okuzenzakalelayo kwamaplatelet omuntu ku-plasma.Ngokuchithwa kwe-alpha granules kuma-platelet, i-PRP ingafihla izici ezihlukahlukene zokukhula, kuhlanganise ne-platelet-derived growth factor (PDGF), i-vascular endothelial growth factor (VEGF), i-fibroblast growth factor (FGF), i-hepatocyte growth factor (HGF), nokuguqula ukukhula kwesici (TGF), okuye kwabhalwa ukuze kuqalise ukuphulukiswa kwesilonda nokukhuthaza ukwanda nokuguqulwa kwamangqamuzana e-endothelial kanye nama-pericyte abe amahlumela e-endothelial.
Izindima ze-PRP zokwelashwa kokukhula kwezinwele ziye zabikwa ocwaningweni oluningi lwakamuva.Uebel et al.bathole ukuthi izici zokukhula kwe-platelet plasma zandisa isivuno samayunithi e-follicular ekuhlinzeni kwempandla yabesilisa.Umsebenzi wakamuva ubonise ukuthi i-PRP inyusa ukwanda kwamaseli e-dermal papilla futhi ibangele uguquko olusheshayo lwe-telogen-to-anagen isebenzisa amamodeli e-vivo kanye ne-in vitro.Olunye ucwaningo luye lwabonisa ukuthi i-PRP ikhuthaza ukuvuselelwa kwezinwele zezinwele futhi inciphise kakhulu isikhathi sokubunjwa kwezinwele.
Kokubili i-PRP kanye ne-platelet-poor plasma (PPP) ihlanganisa ukugcwalisa okuphelele kwamaprotheni e-coagulation.Ocwaningweni lwamanje, ithonya le-PRP ne-PPP ekukhuleni kwezinwele kumagundane e-C57BL/6 laphenywa.I-hypothesis yayiwukuthi i-PRP ibe nomthelela omuhle ekukhuleni kobude bezinwele kanye nokwanda kwenani lezinwele zezinwele.
Izilwane zokuhlola
Sekuphelele amagundane angama-50 anempilo e-C57BL/6 amaduna (amasonto ayi-6 ubudala, angama-20 ± 2 g) atholwe eSikhungweni Sezilwane Zaselabhorethri, i-Hangzhou Normal University (Hangzhou, China).Izilwane zaziphakelwa ukudla okufanayo futhi zagcinwa endaweni eqhubekayo ngaphansi komjikelezo wokukhanya nobumnyama ongu-12:12-h.Ngemuva kweviki le-1 lokujwayela, amagundane ahlukaniswe ngokungahleliwe abe amaqembu amathathu: iqembu le-PRP (n = 10), iqembu le-PPP (n = 10), neqembu lokulawula (n = 10).
Iphrothokholi yocwaningo yagunyazwa ikomiti lesikhungo lezimiso zokuziphatha locwaningo lwezilwane ngaphansi koMthetho Wocwaningo Lwezilwane Nemithethonqubo Esemthethweni e-China.
Isilinganiso sobude bezinwele
Ezinsukwini eziyi-8, eziyi-13, neziyi-18 ngemuva komjovo wokugcina, izinwele eziyi-10 kugundane ngalinye zakhethwa ngokungahleliwe endaweni okuhlosiwe.Izilinganiso zobude bezinwele zenziwa emikhakheni emithathu kusetshenziswa isibonakhulu se-electron, futhi isilinganiso sazo savezwa njengamamilimitha.Izinwele ezinde noma ezilimele azifakwanga.
I-Hematoxylin ne-eosin (HE) staining
Amasampula esikhumba se-dorsal ahlinzwa ezinsukwini eziyi-18 ngemuva komjovo wesithathu.Ngemuva kwalokho amasampula alungiswa ku-10% ye-formalin engathathi hlangothi efakwe kupharafini, futhi asikwa abe ngu-4 μm.Izingxenye zabhakwa amahora angu-4 ukuze zisuswe upharafini ku-65 °C, zicwiliswe ku-ethanol ye-gradient, bese zigcotshwa nge-hematoxylin imizuzu emi-5.Ngemva kokuhlukaniswa ngo-1% we-hydrochloric acid alcohol, izigaba zafakwa emanzini e-ammonia, zigcotshwe nge-eosin, futhi zihlanjululwe ngamanzi acwecwe.Ekugcineni, lezi zigaba zaphelelwa amanzi nge-ethanol ye-gradient, yahlanjululwa nge-xylene, yafakwa nge-resin engathathi hlangothi, futhi yabhekwa kusetshenziswa i-microscopy ekhanyayo (i-Olympus, i-Tokyo, Japan).
Isikhathi sokuthumela: Oct-12-2022