Amaphuzu alandelayo kufanele aqashelwe:
1) Lapho wenza uhlolo lwamaseli okulima, naka ukusebenza kwe-aseptic, vala inzalo ama-reagents (isixazululo sokuhlukanisa, isixazululo sokugeza, njll.) kanye nezinsimbi.Lo msebenzi kufanele wenziwe ngochwepheshe ukuze kuqinisekiswe ukuhlangana komsebenzi.
2) Izinga lokushisa le-Centrifugation livamise ukuba kuzinga lokushisa elilingana negumbi (2~25℃).
3) Ngokuvamile uma kuhlukaniswa amaseli e-mononuclear (PBMC) nge-Ficoll, inani leseli elibomvu legazi lincane, elingenza ukunemba kokuhlolwa.Ngakho i-lysate ingasetshenziswa (ezinye zidinga ukuvalwa inzalo), lawula isikhathi se-lysis futhi uvimbele ukuthinta umsebenzi wamaseli e-mononuclear.
4) Qaphela ngokuphinda umise i-centrifugation engaphinda kabili ukuhlanjululwa.